Spytamlyase Essay

1008 words - 5 pages

Spytamylase 
el 
 
 
 

 
 
 
 
Formål 
I denne øvelse vil vi undersøge hvorledes enzymet spytamylase (engelsk: salivary amylase) 
katalyserer fordøjelsen af stivelse, som er en meget vigtig ingrediens i mange af vores basis 
ernæringsmidler. 
 
 
 
 
 
 
 
 
 
 
 
 
 
 

Teori 
(Når vi spiser, får vi energi fra tre forskellige typer af organiske stoffer, som er kulhydrater, 
proteiner og fedt.)  
 
Hvad er kulhydrat? 
Kulhydrater er, sammen med fedt og protein, en af kostens vigtigste stoffer.  
Kulhydrater er sukkerstoffer som består af kemiske forbindelser, der indeholder meget 
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Navn:

Størrelse

enzym

Glukose (mælk(

monosakkarid

%

fruktose (fruktose)

monosakkarid

%

galaktose
(druesukker)

monosakkarid

laktase

maltose (maltsukker)

disakkarid

maltase

laktose (mælk)

disakkarid

laktase

amylose (stivelse,
Kartofler )

polysakkarid

amylase

 
(dette er en oversigt af hvilke kulhydrater der bliver nedbrudt) 
 
Enzymer: 
enzymer er proteiner, som dannes af levende celler, der også katalyserer kemiske 
reaktioner (forøger hastigheden) ​
.  
 
Enzymernes funktioner. 
 
Enzymerne hjælper alle kropslige funktioner. Fordøjelses enzymer nedbryder føde ( mad ) 
for bevarelse i lever og muskler. Den opbevarede energi bliver så  senere omdannet til 
andre enzymer til kroppens brug når det vil være nødvendigt.  
 
 
 
 
 

 
Hypotese 
Vi bruger spytamylase til at nedbryde stivelse i kroppen, og derfor ville enzymet  tilpasset sig 
til kroppens temperatur, og det vil betyde at den arbejder mest aktivt ved ca. 37˚ C altså 
kroppens temperatur. Og da molekyler bevæger sig meget hurtigt ved høje temperaturer og 
langsomt ved lave, det ville så siges at enzymaktiviteten vil bevæge sig i takt med når 
temperaturen stiger eller falder.  
Når temperaturen er lavt, ville enzym aktiviteten udeblive i det laveste, og når temperaturen 
er meget høj vil den enten tag skade eller blive hæmmet. Det vil så sige at i vores forsøg 
ville vi forvente at reaktionstiden i de forsøg som bliver udsat for høje temperaturer være 
lavere end de forsøg som bliver udsat for lavere temperaturer. 
 
 
 
 
 
M
​ aterialer og apparatur 
 
● Lille bægerglas 
● Pipetter 
● Reagensglas 
● Stopur 
● 5mL stivelse 
●  ​
0,5 mL spyt  
 
 
 
● Fremgangsmåde 
Enzymopløsning 
Vi skulle samle 5 mL spyt i et lille baægerglas, og hvor vi derefter tilsatte ca. 5 mL vand, for 
at det let kan pipetteres.  
 
Enzym assay 
 
(Man kan undersøge hvor hurtigt enzymet nedbryder stivelsen ved at lave et såkaldt enzym 
assay): 
  
Til t = 0 min blandede vi 5mL stivelse med 0,5 mL spyt i et reagensglas.  
Hvor efter vi brugte et stopur til at måle hvor lang tid der gik før opløsningen havde mistet 
farve som den standardopløsning vi havde. 
 
Derefter skulle vi så planlægge og udføre nogle forsøg, som kunne besvare disse 
spørgsmål: 
1: Hvordan afhænger reaktionstiden af enzymmængden? 
2: Hvordan afhænger reaktionstiden af temperaturen? 
3: Har nogle personer højere enzymaktivitet i deres spyt end andre? 

 
 
 

 

 
 
 
 
 
Resultater 
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