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Investigation Of How The Concentration Of Catalase Enzyme Affects The Rate Of Reaction

1457 words - 6 pages

Investigation of How the Concentration of Catalase Enzyme Affects the Rate of Reaction

Aim:

To find out how the concentration of Catalase Enzyme will affect the
enzyme activity and the rate of reaction towards Hydrogen Peroxide. (H
O )

Prediction:

I predict that with the higher concentration of enzyme, the likelihood
of it breaking down molecules will be greater because there will be
more enzymes to work at the substrate and the chances of it colliding
will be higher making the activity time quicker.

Equipment:

· Syringe

· Measuring Cylinder (×2)

· Knife

· Blender

· Beakers (×2)

· Balance

· ...view middle of the document...

I have decided to do a Preliminary Experiment because it will be a
rough experiment to help me with the real one. It will be like a trial
run so that if anything goes wrong in this one then it will tell me
what I'll need to change for the actual experiment if I need to.

Firstly I weighed 100g of potato and used 100cm3 of water to be
blended together by an electric blender. This was placed in a beaker
and this was our Catalase. To use different concentrations we started
off with the 10ml of Catalase added to 10ml of Hydrogen Peroxide.
After 20 seconds we were supposed to measure how much water and oxygen
(froth) was produced but the froth had already gone over the top of
the 100 ml measuring cylinder. As this was obviously too much we
started to try a trial run using only 5ml of Catalase to 10ml of
Hydrogen Peroxide. This amount suited the experiment.

Results for Preliminary

Concentration Of Catalase Enzyme

Experiment Attempts

Catalase

Water

1st

(100%) 10ml

(0%) 0ml

ml

(100%) 5ml

(0%) 0ml

ml

(80%) 4ml

(20%) 1 ml

ml

(60%) 3 ml

(40%) 2ml

ml

(40%) 2ml

(60%) 3ml

ml

(20%) 1 ml

(80%) 4ml

ml

Conclusion for Preliminary Experiment

During the preliminary experiment I realised that 10ml of the Catalase
added to the 10ml of Hydrogen Peroxide would be far too much therefore
I changed the amount of Catalase to 5 ml which became much more suited
to the experiment because after 20 seconds when we measured it, it had
not gone over the top of top of our measuring cylinder unlike 10ml
did.

We came to the decision that the enzyme Catalase found in the potato
would be better off liquidised as it would be easier for Hydrogen
Peroxide to collide with the Catalase because it is in smaller
measuring particles. Also the measuring cylinder we were using was
very big and it would be more reliable if we used a smaller measuring
cylinder so that the result is more accurate.

Safety Note

· Care should always be taken when handling chemicals therefore make
sure you are careful using the Hydrogen Peroxide as it can be
irritable and harmful.

· Be careful when using water baths because they can reach dangerously
high temperatures

· Use the blender appropriately as mishandling it could cause harm.

Method

1. Using a balance weigh out 100g's of potato (used as the Catalase as
it contains it.

2. Put it into a blender adding 100ml of water to it and blend it
together until it liquidizes.

3. Put some Hydrogen Peroxide into a beaker and put into a water bath
to heat it up until it reaches the Catalase's optimum temperature for
it to work at its best.( The optimum temperature came to my attention
whilst using my secondary resources to back up my...

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