This website uses cookies to ensure you have the best experience. Learn more

Investigating The Optimum Ph Bacterial And Mammalian Amylase Digests Starch Solution

1647 words - 7 pages

Investigating the Optimum pH Bacterial and Mammalian Amylase Digests Starch Solution

[IMAGE]

Biology

Coursework

Monique Noel 12T

Finding the Optimum pH Bacterial and Mammalian

Amylase Digests Starch Solution.

Introduction

I am investigating the optimum pH bacterial and mammalian amylase
digests starch solution. To do this I will conduct an experiment using
bacterial and mammalian amylase, subjecting them to varying pH levels
and record at which pH level the enzyme digests starch solution most
efficiently.

My hypothesis: I predict that mammalian amylase will have an optimum
pH that is more neutral then ...view middle of the document...

In the case of this particular experiment each enzyme has an
optimum pH level at which it works best at. When an enzyme is
subjected to extreme changes in pH it can alter the shape of the
active site this happens because a protein is made in four stages. The
third stage is called the tertiary structure. At this stage the
protein forms its three dimensional shape. The three dimensional shape
is held together by hydrogen and ionic bonds. pH alters hydrogen
concentrations and extreme pH levels are consistent to the changes on
the protein. This is because the ionic bonds cannot be maintained and
the active site is disrupted, the enzyme will denature meaning the
active site changes shape and the substrate no longer fits. The change
in shape is not permanent and can be returned back to shape.

From the background information I suggest that mammalian amylase has
an optimum pH of around 7 as it is produced in the mouth to break down
food there for it can not be to acidic or alkali otherwise it would
irritate the inside of the mouth. For bacterial amylase I hypothesise
that it has a more acidic pH as it is used for digestion of bacteria
near the stomach, the stomach is more acidic then the mouth. I will
carry out the following experiment to find evidence for my
predictions.

Method:

First experiment using bacterial amylase:

pH buffers 2-11 3cm³ of each

Bacterial amylase 0.1% 30cm³

Starch solution 2.5% 150cm³

Potassium Iodate

1. Prepare 3 spotting tiles by dropping 2 drops of potassium iodate
onto each concave of the spotting tile.

2. Using a 2cm³ syringe add 1cm³ of bacterial amylase and 1cm³ of pH 2
buffer to a test tube. Do this 3 times into separate test tubes and
place all 3 into a test tube rack and number the test tubes 1-3.

3. Using a 10cm³syringe add 5 cm³ of starch solution into test tube 1
and start the stop clock

4. Every 10 seconds take a sample with a pipette and add one drop to
the potassium iodate. (Repeat this step until the potassium iodate
does not changed in colour).

REPEAT WITH ALL 3-11 PH BUFFER

Second Experiment using mammalian amylase:

pH buffers 2-11 3cm³ of each

Mammalian amylase 30cm³ (source from your own saliva)

Starch solution 1.25% 150cm³

Potassium iodate

1. Prepare 3 spotting tiles by dropping 2 drops of potassium iodate
onto each concave of the spotting tile.

2. Using a 2cm³ syringe add 1cm³ of Mammalian amylase and 1cm³ of pH 2
buffer to a test tube. Do this 3 times into separate test tubes and
place all 3 into a test tube rack and number the test tubes 1-3.

3. Using a 10cm³syringe add 5 cm³ of starch solution into test tube 1
and start the stop clock.

4. Every 10 seconds take a sample with a pipette and add one drop to
the potassium iodate. (Repeat this step until the potassium iodate
...

Other Papers Like Investigating The Optimum PH Bacterial And Mammalian Amylase Digests Starch Solution

Investigating The Effects Of An Abiotic Factor On The Frequency And Distribution Of A Freshwater Invertebrate

926 words - 4 pages Investigating the Effects of An Abiotic Factor on the Frequency and Distribution of a Freshwater Invertebrate (i) Planning ------------ Introduction ============ Before a complex biological study can be planned and formulated, the terminology in the title above must be clarified. The investigation requires a sound knowledge of ecology, which essentially is the study of organisms, whether they be

German Attitudes Toward The Jews And The Final Solution

1325 words - 6 pages German Attitudes Toward the Jews and the Final Solution There are those that claim that Hitler’s conscious personal hatred of the Jews, his unique and central role in the rise of Nazi Germany were fundamental in the development of the anti-Jewish policies that emerged leading to the final solution. However, there is strong evidence to suggest that the anti- Jewish feeling in Germany reflected a much stronger, widespread

Investigating If The Number Of Leaves Affect The Water Loss From The Previt And The Stem And Leaves

644 words - 3 pages Investigating if the Number of Leaves Affect the Water Loss From the Previt and the Stem and Leaves Ideas. · The amount of water in leaves. · The thickness of the stem. · The number of leaves. · Size of the roots. · Temperature. · The amount of leaves that are left on the stem. · And finally the sizes of the leaves. AIM. Our Aim is to find out if the number of leaves affects the water

Domain Tasting Problem in 2003 and the Solution

678 words - 3 pages . The Defense Department realized that in the future they need powerful computers for coordination and control. The Defense Department funded a research at leading universities and institute with the goal of designing a worldwide network that could remain operational even the network were sabotage by the military enemy. The researchers were determined the best solution to accomplishing their goal was to create networks that do not require a central

Investigating the Effect of Concentration on the Speed of the Reaction Between Hydrochloric Acid and Sodium Thiosulphate

1244 words - 5 pages Investigating the Effect of Concentration on the Speed of the Reaction Between Hydrochloric Acid and Sodium Thiosulphate Prediction: My prediction is, the higher the concentrations, the faster the reaction will occur in my experiment to make the x on the sheet on paper disappear. When there is more concentration, the molecules gain kinetic energy & the process of collision occurs. This causes the reaction to happen

investigate the action of saliva and hydrochoric acid in two carbohydrate solution

976 words - 4 pages reducing sugar. In spite of that, result obtained from Benedict’s solution and iodine test for solution B are negative and positive respectively, which as a result that solution B is a starch. Because starch is a polysaccharide, a complex sugar which completely composed of a long chain of glucose monomers, solution B has a more complex structure compare to solution A which is just a monosaccharide, simple sugar. 9) To sum up, solution A is a reducing sugar and solution B is a starch. Starch can be broken down into reducing sugar by hydrolysis in the present of amylase or hydrochloric acid.

"Napoleon Bonaparte Investigating And Researching Task" - Investigate And Research The Life Of Napolean And Discuss The Major Events In His Life And Mistakes

1261 words - 6 pages Napoleon Bonaparte Investigating and Researching Task Napoleon Bonaparte Background Napoleon Bonaparte was born in August the 15th 1769 on the island of Corsica. He was the second of eight children of Carlo (Charles). Since Corsica had recently become a part France after the king bought it off the Italians, his parents were then Italian and he French. At the age of nine, Napoleon was sent to a school in France. He spent seven years in

Determining the Activation Energy of the Reaction Between Bromide Ion and Bromate Ion in Acid Solution

572 words - 3 pages Determining the Activation Energy of the Reaction Between Bromide Ion and Bromate Ion in Acid Solution Objectives: By reacting Potassium Bromide and Potassium Bromate in the acid solution in varies temperature, we can then determine the Activation Energy of the reaction in different temperature through the equation:[IMAGE]. Results: Experiment Temperature Time t/s ln 1/t Temperature K 1/K

Using Material from Item B and Elsewhere, Assess the Strengths and Limitations of Overt Participant Observation as a Means of Investigating Police Attitudes

1090 words - 5 pages Using material from Item B and elsewhere, assess the strengths and limitations of overt participant observation as a means of investigating police attitudes Overt participation is usually a method of research carried out by those from a non-interactionist perspective, even though it is something that does focus on some interaction. When investigating police attitudes we mean attitudes of the police towards those who commit crime or are those

“Here's to Alcohol: the Cause of, and Solution to, All of Life's Problems.”

1924 words - 8 pages “Here's to alcohol: the cause of, and solution to, all of life's problems.” The United States is very different from many other countries in many different ways. One of these ways is the way that we look at drinking and the age at which we allow people to legally consume alcohol. Our country was founded on Puritan beliefs, which doesn’t surprise me as to why we have such a high drinking age when compared to other countries. Many of which

Experiment to test the time neutrase and milk solution takes to clear

595 words - 3 pages What is the experiment about?The experiment is about finding the time it takes for a solution of neutrase and milk to clear when using different concentrations of neutrase.Why did I choose to experiment on how the concentration of neutrase effects the time a milk solution takes to clear?I could have chosen to test how the reaction times differed at different temperatures, but I chose to experiment on the effect different concentrations of

Related Essays

Effects Of Different Ph Level On Amylase In Starch

2961 words - 12 pages through. The more light that passes through the test tube means more starch was broken down into glucose. (Luesse, 2012). We hypothesized that the enzyme amylase will be most optimum around the pH of 7 and less active away from neutrality. Bioinformatics is an online database by which, scientists can look up any known protein sequence and protein function. It focuses on the sequence and structure of DNA, RNA, and protein. (Goto et. al., 1998

The Efeects Of Salivary Amylase On Starch Hydrolysis Under Different Ph Levels

746 words - 3 pages ) by maltase, another enzyme.These reactions however occur only under specific environental conditions provided within the mouth. This experiment assesses at which pH level salivary amylase works best under, in the breakdown of starches, with the pH of 7 (average pH level found within the mouth) hypothesized to be more efficient in starch hydrolysis by salivary amylase.Materials and Methods While waiting for the spectrophotometer to warm up at a

The Misuse Of Antibiotics And Bacterial Resistance

2643 words - 11 pages The Misuse of Antibiotics and Bacterial Resistance Samantha Onda Penn State Worthington Scranton Abstract The growing issue of bacterial resistance to antibiotics is growing more rapidly than anticipated. Numerous factors contribute to this growing issue and it creates a major concern for society today. The main factor in this growing issue is the continuous misuse and overuse of antibiotics. For example, people use them by prescribing

Investigating The Length Of Words In Magazines And Newspapers

684 words - 3 pages Investigating the Length of Words in Magazines and Newspapers Introduction In this piece of coursework I am going to look at the differences between articles from newspapers and magazines. I will be looking at the length of the words in each of the articles to see which of them has the longest words. Plan I am going to take a 100 word article from both newspaper and magazine. The article that I have chosen