Hydrolysis Of Sucrose Essay

811 words - 4 pages


Chemical Kinetics involves the determination of velocity or rate of a chemical reaction. The rate reaction depends on the nature of the reactants , the concentration of the reactants, the temperature, and the presence of a catalyst. The concentration of species on the reaction rate are summarized by an equation called the Rate Law for the reaction. Effect of temperature on the reaction rate leads to the energy required for a reaction. Also to determine the rate law, it should be done experimentally. The hydrolysis involves the reaction of sucrose with water in the presence of H+ to form glucose and fructose. Using a ...view middle of the document...

0mL of 3M HCl in the 50mL graduated cylinder. As soon as Sucrose and HCl solutions were mixed, the acid-catalyzed hydrolysis of sucrose begins. We timed the reaction carefully from the moment the two solutions were mixed. After 1minute of the reaction has lapsed, we pipet 2.00mL aliquot of the reaction mixture into test tube 1. The NaOH stops the reaction. We then made sure that the solution is fully mixed. Procedure was repeated for the rest of the tubes at the end of 2,4,6,8,10,12,15 and 20 minutes total reaction time. The concentration of Sucrose in solution at various stages of the reaction was determined indirectly. First, we measured the concentration of fructose and glucose formed spectrophotometrically by reacting them with 3,5-dinitrosalicylate reagent (3,5-DNS). Both sugars reacted with the reagent to form a colored product that absorbed strongly at 540nm. The absorbance of the solution was measured and the concentration of fructose and glucose was determined from Beer’s Law (A=elc). The value of el for the product was 1200L/mol, which was used for our calculations. The concentration of glucose + sucrose that was measured in the Beer’s Law experiment was then used to calculate the concentration of glucose + fructose formed in our reaction. Since sucrose has two products, glucose and fructose, the moles/L consumed is determined with the formula: [S]^consumed=0.5[glucose +fructose formed] . To obtain the [S]f,...

Other Papers Like Hydrolysis of Sucrose

Biological Significance of Monosaccharides Essay

884 words - 4 pages relative sweetness. Large amounts of sucrose are consumed all around the world, especially in countries such as the Sudan, Egypt and Libya to name a few. In these countries tea is taken frequently with plenty of sucrose. Many physicians believe that large amounts of sugar increase the risk of disease conditions including diabetes mellitus. Sucrose also causes dental caries, tooth decay. On hydrolysis, in the presence of an enzyme (invertase or sucrase) , sucrose yields equal amounts of glucose and fructose. The mixture of glucose and fructose is called invert sugar.

Glucose Essay-What Is It

669 words - 3 pages most saps of trees, most fruits and fruit juices. Glucose is the most common sugars. It is also knows as dextrose or grape sugar. Glucose makes up the rubbery part of plants. Starch and glycogen are polymers of glucose. In humans and animals, glucose is needed to give energy for muscles and other processes of the body needed to use energy. Men and women get glucose from starch, glycogen, sucrose, and lactose, and then they are broken down

The Function of Proteins Are Linked to Their Shape

943 words - 4 pages , animals use muscle contraction. Muscle fibres are composed of two protein filaments, myosin which is a thick filament and actin which is thinner. Actomyosin cross-bridges can form between the two which move relative to one another on hydrolysis of ATP drawing actin into myosin. This sliding filament theory shows how a sarcomere contracts. This contraction is used in a variety of applications including constriction or dilation of blood vessel to

Biology Lab Report

2636 words - 11 pages -products from sugar metabolism lowered the pH of the medium (refer to Table 1). The biochemical test results for dextrose, lactose, maltose, and sucrose for Haloferax volcanii are positive, which means that the culture medium appeared yellow (refer to Table 1). The biochemical test result for starch hydrolysis for Halobacterium salinarum is positive and is indicated by colourless areas around growth, whereas for Haloferax volcanii is negative


2357 words - 10 pages Molecules associated with living organisms are organic. These include nucleic acids, fats, sugars, proteins, enzymes and many fuels. * DNA * table sugar or sucrose, C12H22O11 * benzene, C6H6 * methane, CH4 Examples of Inorganic Compounds Inorganics include salts, metals, substances made from single elements and any other compounds that don't contain carbon bonded to hydrogen. * table salt or sodium chloride, NaCl * carbon

Carbohydrase Market - Global Industry Analysis, Size, Share, Trends and Forecast, 2013 - 2019

965 words - 4 pages types of sugar syrups such as fructose and glucose. Moreover, carbohydrase enzymes are used for the production of prebiotics and isomaltulose. Prebiotics and isomaltulose is a sweetener and is used as a substitute for sucrose for improving the properties of juices and wines and also to reduce the level of lactose in milk. Moreover, carbohydrase enzymes are widely used as ingredients and processing aids in distinct industries. Furthermore, the two

Lab Report 1

2869 words - 12 pages distilled water + 1 cm3 of Benedict reagent | E | 5 cm3 1% glucose + 5 cm3 distilled water + 1 cm3 of Benedict reagent | F | 5 cm3 1% sucrose + 5 cm3 distilled water + 1 cm3 of Benedict reagent | G | 5 cm3 0.01% glucose + 5 cm3 distilled water + 1 cm3 of Benedict reagent | H | 5 cm3 W juice + 5 cm3 distilled water + 1 cm3 of Benedict reagent | I | 5 cm3 Y juice + 5 cm3 distilled water + 1 cm3 of Benedict reagent | J | 5 cm3 Z juice + 5

Ap Biology

2581 words - 11 pages the term amino acid signify about the structure of such a molecule? 6. What chemical change occurs to ATP when it reacts with water and releases energy? Chapter 5: The Structure and Function of Large Biological Molecules 1. Review and know these terms: polymer, monomer, enzyme, dehydration reaction, hydrolysis 2. What are the four main classes of large biological molecules? Which class does not consist of polymers? 3. How

Chemistry Quantitative Analysis

2570 words - 11 pages about 5ml of glucose solution in a test tube with about 2ml Benedict’s reagent which is blue. The test tube is then left to stand in a hot water bath for 5 minutes. Glucose will cause an orange/red precipitate if present. Test food for non-reducing sugar Place about 5ml of sucrose solution and 2ml of hydrochloric acid in a test tube, then place in a water bath and gently boil for 5 minutes. Remove the test tube and allow it to cool. When cool

Variety of Mysterious Microorganisms

2527 words - 11 pages can identify if the organism can ferment lactose, if it has the ability to reduce litmus, if it causes the accumulation of casein, and if the organism has the capability to breakdown casein by hydrolysis. The main ingredients within the medium are skim milk and azolitmin. The skim milk contains nutrients that are essential for growth of the organism. It also contains lactose that will help identify the utilization of fermentation. The milk also

Mordern Medicines

3715 words - 15 pages affecting equilibrium – Le Chatelier’s principle; ionic equilibrium – ionization of acids and bases, strong and weak electrolytes, degree of ionization, concept of pH. Hydrolysis of salts (elementary idea), buffer solutions, solubility product, common ion effect (with illustrative examples). Unit VIII: Redox Reactions Concept of oxidation and reduction, redox reactions, oxidation number, balancing redox reactions, applications of redox reactions

Related Essays

Substitution Of Asp 309 By Asn In The Arg Asp Pro (Rdp) Motif Of Acetobacter Diazotrophicus Levansucrase Affects Sucrose Hydrolysis, But Not Enzyme Specificity

2783 words - 12 pages Biochem. J. (1999) 337, 503–506 (Printed in Great Britain) 503 Substitution of Asp-309 by Asn in the Arg-Asp-Pro (RDP) motif of Acetobacter diazotrophicus levansucrase affects sucrose hydrolysis, but not enzyme specificity ; ; ; ; Frank R. BATISTA, La! zaro HERNANDEZ1, Julio R. FERNANDEZ, Juan ARRIETA, Carmen MENENDEZ, Rau! l GOMEZ, ; Yanet TAMBARA and Tirso PONS Centre for Genetic Engineering and Biotechnology (CIGB), P.O. Box 6162

Hydrolsis Of Sucrose Essay

2183 words - 9 pages Chemistry 102 - Experiment 5 Chemical Kinetics: The Acid-Catalyzed Hydrolysis of Sucrose INTRODUCTION Chemical kinetics involves the determination of the velocity or rate of a chemical reaction. The rate of a reaction depends on the nature of the reactants, the concentration of the reactants, the temperature, and the presence of catalysts. The effect of the concentration of various species on the reaction rate is summarized by a general

Investigating The Activity Of An Enzyme

794 words - 4 pages increased because this would enable the sucrose to be broken via hydrolysis more quickly. The number of enzyme solutes contained in lower concentrations would be less, meaning the initial velocity rate would be slower. Ultimately there will always be a limit in rate of reaction deemed by either substrate or enzyme concentration. This may over be overcome finding an optimum, termed V max. Meaning there would be no way the

Investigating How Changing The Concentration Of Sucrose Affects Its Reaction Time

2114 words - 9 pages rate of a chemical reaction without directly taking part in the reaction. For substrates to be broken down into their The necessary components of a enzyme reaction are an enzyme and a substrate. In the case of my experiment, the substrate is sucrose and the enzyme is Sucrase. During the reaction, sucrase breaks sucrose into its two monosaccharide constituents, glucose and fructose, by catalyzing the hydrolysis reaction