This website uses cookies to ensure you have the best experience. Learn more

Effects Of Different Ph Level On Amylase In Starch

2961 words - 12 pages

Effects of Different pH Level on Amylase in Starch

Introduction
Amylase, a family of proteins that differ in isoforms, is a digestive enzyme found in saliva and pancreatic fluid that helps digest starch into simple sugars. (Scannapieco et. al., 1993). Amylase is the first step in digesting starch, which is used for the intake of carbohydrates or energy in humans. (Butterworth et. al., 2011). Amylase functions in the hydrolysis of starches, which produces glucose monomers. (Karp, 2010). This is essential in glycolysis and the harvesting of ATP. Since amylase is also a protein that performs enzymatic reactions, the secondary and tertiary structures are affected by external stimuli like ...view middle of the document...

(Luesse, 2012). The amylase protein sequence was analyzed by comparing it to other eukaryotic organism’s amylase proteins and looking at their similar protein sequences.

Figure 1: Experimental Test Tubes. Test tubes 0-15. 0(blank), 1-3(pH2), 4-6(pH 6), 7-9(pH 7), 10-12(pH 8), 13-15(pH 12). Test tubes 1, 4, 7, 10, and 13 are at zero minute, 2, 5, 8, 11,and 14 are at five minutes, and 3, 6, 9, 12, and 15 are at ten minutes.
Materials and Methods

In the experiment, sixteen 10ml test tubes were used to conduct this experiment. They were labeled 0-15 with zero being the blank and the rest being different pH levels as follows: 1-3 pH two, 4-6 pH six, 7-9 pH seven, 10-12 pH eight, and 13-15 pH twelve. The sets of three are for times zero, five, and ten (Figure 1). Then 2 ml of IKI is added to all of the test tubes, 0-15. The IKI is used to stop the reaction and see how much of the starch is broken down by amylase by staining the starch. In the blank (0), 2 ml of water is added to see how much light passes through when there is no starch present in the media. (Luesse, 2012). The digest solution is made in five 30 ml tube with 5 ml of 0.1% starch and 8 ml distilled water in all of the tubes, and 2 ml of each pH buffer’s 2, 6, 7, 8, and 12 in one of the tubes. The tubes should be labeled 2, 6, 7, 8, and 12 to differentiate the different digest solution. This solution does not contain amylase so the reaction has not yet started. This digest solution is what makes the controls for each pH level. 2 ml of the digest solution for each pH level is added to 1, 4, 7, 10, and 13 respectively to create a control that is without the enzyme amylase. Have a stop watch in hand and add 5 ml of amylase solution to the each of the five digest solutions and start the stop watch. This is when the reaction started and the time should be started.
After five minutes have passed, 2 ml of the new solution should be removed from each pH level to the five minute test tubes or 2, 5, 8, 12, and 14. This represents five minutes of the reaction that has proceeded and is frozen when added to the test tubes. (Luesse, 2012). After ten minutes have passed 2 ml of the reaction solution is added again, but in the ten minute test tubes, 3, 6, 9, 12, and 15 respectively for each pH level. All the samples are now collected and 2 ml of water is added to make a total volume of 6 ml. The spectrophotometer was then used to see how much of the starch is broken down for each pH and time.
Bioinformatics was used to find amylase’s protein sequence. This was done by using the online database of the National Center for Biotechnology Information. To find the protein amylase in the system, human amylase was put into the search bar and was narrowed down to only search for proteins. When the results came up, an amylase sequence of 511 amino acids was found and saved for further used. Next, three other organism’s amylase protein sequences were found to compare to the human amylase protein...

Other Papers Like Effects of Different Ph Level on Amylase in Starch

Soil Ec And Ph Using 3 Types Of Extractant Solutions On Different Soil Samples

1562 words - 7 pages ------------------------------------------------- Soil EC and pH using 3 types of extractant solutions on different soil samples ------------------------------------------------- Ben Vincent ------------------------------------------------- AGR2IlS subject coordinator: Dr Gary Clack ------------------------------------------------- INTRODUCTION ------------------------------------------------- pH is in general terms the about the

The Action Of Saliva On Starch

1107 words - 5 pages Experiment 9. The action of saliva on starch Study the flow chart on p. 9.02 for a few minutes to gain an idea of the outline of the experiment. (a) Prepare a water bath by using a Bunsen burner to heat some water in a beaker on a tripod and gauze till it boils; then turn the flame down to keep the water just boiling. While waiting for the water to boil, carry on from (b). (b) Label eight test-tubes 1 - 8 and in tube 1 collect saliva

The Effects of Different Governments on Society Portrayed in Lord of the Flies

683 words - 3 pages by Jack, who unlike Ralph represents a different form of government, a dictatorship. Jack is a dictator, who rules with fear and sheer power, and when in he is in power, life on the island becomes a communist society. Jack’s rise to power is a result of him creating a “fear of the unknown”, and creating a belief that only he could be trusted to handle their fears. This is evident in that, Jack treats the “beastie” as the unknown

Cloning and Expression of Α-Amylase Gene from Bacillus Subtilis in Pichia Pastoris and Escherichia Coli

1774 words - 8 pages enzymes available in market, α-amylase has received a special attention in commercial production due to its widely used applications. α-Amylase contributed to 50% of the world enzyme production and has a great importance in many industries such as in food processing, laundry and also in pharmaceutical (Asgher et al. 2007). α-Amylase enzyme acts on α-1,4 glycosidic bonds in starch substrate backbone leading to the formation of soluble maltodextrins

The Effect Of Different Level Of Npk 15:15:15 Fertilizer On The Vegetative Growth And Yield Of Tomato (Lycopersicon Esculentum) In Yandev. Area Of Benue State A Project Research Work Submitted To...

5426 words - 22 pages therefore to search for the best production (yield) in this area through identification of ideal levels of fertilizer that will enhance yield in tomato production, since fertilizers are very costly and scarce. 1.3 OBJECTIVES OF THE STUDY The study has instituted the broad objective to determine the effect of different level of N.P.K 15:15:15 fertilizer on the growth and yield of tomato. However the specific objective established are as follows

Biology Lab Report On The Effects Of Different Bio-Toxins On E. Coli

1081 words - 5 pages in a lab. Chlorine/bleach, on the other hand, appears to vary but stay within a particular range, as well as have the highest zones of inhibition out of the three. Other studies have found cases of evolved triclosan resistance in naturally occurring populations, and have described the mechanisms by which bacteria can get around the effects of triclosan. The concerns associated with the development of triclosan resistance are based on several

How Do the Effects and Responses of Earthquakes Differ in Countries of Different Economic Status?

651 words - 3 pages How do the effects and responses of earthquakes differ in countries of different economic status? On the 12th may 2008 Sichuan in China suffered from an earthquake, although China is an NIC Sichuan is one of the poorest states and so the responses and effects of the earthquake will be very different to those in an MEDC such as the 1995 Kobe earthquake. Perhaps the most contrasting effect of the earthquake is the death rate; 6 500 in Kobe and

Effects of Different Dietary Vitamin C

4955 words - 20 pages Marsland Press Journal of American Science 2009:5(3) 31-40 Evaluations of the Effects of Different Dietary Vitamin C levels on the Body Composition, Growth Performance and Feed Utilization Efficiencies in Stinging Catfish, Heteropneustes fossilis (Bloch, 1792) Md. Jobaer Alam 1, Md. Ghulam Mustafa 1, Md. Abdul Khaleque 2. 1 2 Department of Fisheries, University of Dhaka, Dhaka-1000, Bangladesh Institute of Food Science and Technology

Outline of the Early Development of a Self-Concept and the Possible Effects of Different Parenting Styles on Children

1939 words - 8 pages cognitively competent than others” (Baumrind, 1991). In addition, “the effects of authoritarian upbringing in early childhood have been found to be more harmful for middle-class boys than girls” (Baumrind, 1991). In addition to differing on responsiveness and demandingness, the parental styles can also be characterised by a third dimension – psychological control. This refers to “control attempts that intrude into the psychological and

Evaluate Effects Of Globalisation On Medias Role In Society

857 words - 4 pages Evaluate the effects of globalisation on the role of the media in society over the past thirty years or so (33 marks) Over the last 30 years’ globalisation has influenced media in many different ways, however these effects can have advantages and disadvantages. One of the effects globalisation has that is visible is that communication has become wide spread. Over the last few years, magazines, newspapers, the internet and television have spread

The Effects Of The Blitz On Everyday Life In Britain

896 words - 4 pages The Effects of The Blitz on Everyday Life in Britain When Adolf Hitler ordered the Luftwaffe to concentrate its bombing efforts on the major cities of Britain in the summer of 1940, in an effort to destroy British moral, the people of Britain were subjected to an almost nightly ordeal of high explosives and incendiary bombs. There are many heroic stories about the Blitz- of exceptional bravery, a product of the so

Related Essays

The Efeects Of Salivary Amylase On Starch Hydrolysis Under Different Ph Levels

746 words - 3 pages ) by maltase, another enzyme.These reactions however occur only under specific environental conditions provided within the mouth. This experiment assesses at which pH level salivary amylase works best under, in the breakdown of starches, with the pH of 7 (average pH level found within the mouth) hypothesized to be more efficient in starch hydrolysis by salivary amylase.Materials and Methods While waiting for the spectrophotometer to warm up at a

Investigating The Optimum Ph Bacterial And Mammalian Amylase Digests Starch Solution

1647 words - 7 pages iodate. (Repeat this step until the potassium iodate does not changed in colour). REPEAT WITH ALL 3-11 PH BUFFER Second Experiment using mammalian amylase: pH buffers 2-11 3cm³ of each Mammalian amylase 30cm³ (source from your own saliva) Starch solution 1.25% 150cm³ Potassium iodate 1. Prepare 3 spotting tiles by dropping 2 drops of potassium iodate onto each concave of the spotting tile. 2

Effect Of Water Temperature On Amylase Using Colorimetric Starch Assay

1540 words - 7 pages Timothy Pratt Wednesday, 12:20 PBIO 1210L Scientific Paper: Effect of Water Temperature on Amylase Using Colorimetric Starch Assay Introduction For plants to function properly, chemical reactions must take place within the cells of each individual plant. Energy is necessary for each reaction to occur. The amount of energy that is necessary for a reaction is called the activation energy. Enzymes are used to reach the correct activation

Effect Of Enzymes On Starch Essay

2133 words - 9 pages (mm) 15 38.8 40 50 Standard Deviation 0 ± 3.03 ± 1.87 ± 4.12 Qualitative data Amylase is milky white in color Petri Dishes contains condensed vapor on the lid Lots more condensed vapor after taking out of incubator Majority of the area is dark purple in color after pouring iodine on the agar Sample Calculations Average diameter The aim of this lab is to compare the results of different concentrations of amylase to starch. In