This website uses cookies to ensure you have the best experience. Learn more

Effect Of Water Temperature On Amylase Using Colorimetric Starch Assay

1540 words - 7 pages

Timothy Pratt
Wednesday, 12:20
PBIO 1210L
Scientific Paper:
Effect of Water Temperature on Amylase Using Colorimetric Starch Assay


For plants to function properly, chemical reactions must take place within the cells of each individual plant. Energy is necessary for each reaction to occur. The amount of energy that is necessary for a reaction is called the activation energy. Enzymes are used to reach the correct activation energy in plants. They lower the activation energy to the correct amount for the reaction to take place. Certain enzymes catalyze certain reactions, which means that many enzymes to operate the cell. On each cell, there is a spot where the substrate ...view middle of the document...


Iodine-Potassium-Iodide (IKI) was used to perform a colorimetric starch assay. The IKI interacts with the starch chains, forming a blue color. The concentration of the starch molecule is shown by how concentrated the blue color is.
In our control enzyme activity assay, we collected ten test tubes, labeled from zero to nine, and placed one drop of IKI into each tube. Next we took 2.5mL of our solution without any enzyme and added it to the test tube labeled zero. We then took a flask of our starch solution and added 1.0 mL, we immediately started the stopwatch and mixed the enzyme into the solution. We removed 2.5 mL of the reaction and placed it into one test tube with IKI in minute intervals. After nine minutes, all test tubes were filled with the enzymatic solution. The more starch in each test tube, the more concentrated the blue color is and vice versa. However this only gave us qualitative data. To obtain concrete, quantitative results, we took the test tubes over to the spectrophotometer. We wiped off each test tube off with a tissue then placed it into spectrophotometer two and recorded the readings. The results are shown in the table below.
After the control, we started the experiment. We collected twenty test tubes and labeled two different sets zero to nine and placed a drop of IKI into each tube. This time we put the flask that contained the solution in water that was forty degrees Celsius. Once the temperature of the solution was the same as that of the water, we collected 2.5mL from the starch solution and placed it into the test tube labeled zero. Then we added the 1.0 mL of amylase into the flask. At minute intervals, we placed 2.5mL of the reaction into a test tube. We again recorded the absorbance of the test tubes in spectrophotometer two. The same process was used for the second set of tubes. The tables below represent the data we collected.


First Control Trial
Average Reaction Rate per Minute: 4.25 (μg/ml)
Time Absorbance Reading (OD at 580 nm) Starch Concentration (μg/ml) Reaction Rate (μg digested starch/min)
0 0.738 67.65 0
1 0.548 50.23 17.42
2 0.538 49.31 0.92
3 0.474 43.44 5.87
4 0.465 42.62 0.82
5 0.444 40.70 1.92
6 0.399 36.57 4.13
7 0.354 32.45 4.12
8 0.335 30.71 1.74
9 0.321 29.42 1.29

Second Control Trial
Average Reaction Rate per Minute: 5.61 (μg/ml)
Time Absorbance Reading (OD at 580 nm) Starch Concentration (μg/ml) Reaction Rate (μg digested starch/min)
0 0.735 67.37 0
1 0.470 43.08 24.29
2 0.459 42.07 1.01
3 0.364 33.36 8.71
4 0.308 28.23 5.13
5 0.296 27.13 1.10
6 0.246 22.55 4.58
7 0.228 20.90 1.65
8 0.213 19.52 1.38
9 0.184 16.86 2.66

First Experimental Trial (40 Degrees Celsius)
Average Rate per Minute: 5.00 (μg/ml)
Time Absorbance Reading (OD at 580 nm) Starch Concentration (μg/ml) Reaction Rate (μg digested starch/min)
0 0.587 53.80 0
1 0.454 41.61 12.19
2 0.321 30.06 11.55
3 0.239 21.90 8.10
4 0.191 17.50 4.40
5 0.135 12.37...

Other Papers Like Effect of Water Temperature on Amylase Using Colorimetric Starch Assay

Effect of Water Pollution on Lake Huron

629 words - 3 pages .  Part of Lake Huron Shoreline The pollution in Lake Huron has a negative effect on people and the economy of the area. Phosphorus levels threaten the safety of drinking water because of the harmful algae blooms. Deficient oxygen levels because of excessive algae growth makes the fish communities unstable and harmful for consumption. It can cause the extinction of a specifies which disrupts nature’s balance and creates harm to

Investigating The Effect Of Temperature On The Structure Of An Enzyme

1659 words - 7 pages Investigating The Effect of Temperature on the Structure of an Enzyme Introduction: For my GCSE Biology assessment I will be investigating the enzyme amylase with the substrate starch. This reaction, which I am going to investigate, is called the protein test for starch. Aim: My intention for this observation is to examine how the enzyme catalyses are affected by changes in temperature. Safety

The Action Of Saliva On Starch

1107 words - 5 pages Experiment 9. The action of saliva on starch Study the flow chart on p. 9.02 for a few minutes to gain an idea of the outline of the experiment. (a) Prepare a water bath by using a Bunsen burner to heat some water in a beaker on a tripod and gauze till it boils; then turn the flame down to keep the water just boiling. While waiting for the water to boil, carry on from (b). (b) Label eight test-tubes 1 - 8 and in tube 1 collect saliva

The Effect of Time and Temperature on Storage of Fresh Pechay

1591 words - 7 pages The Effect of Time and Temperature on Storage of Fresh Pechay I.Introduction A. Objectives The study is performed to discuss the effect of time and temperature on the storage of fresh pechay. It is also done to explain the difference of low and high temperature on the storage fresh pechay. Lastly, to explain other factors related to time and temperature that may affect the storage. Through conducting this study, the proper storage

Investigating The Effect Of Temperature On The Movement Of Pigment Through Cell Membranes

1017 words - 5 pages Investigating the Effect of Temperature on the Movement of Pigment through Cell Membranes INTRODUCTION: The aim of experiment is to prove that temperature effects on a proteins in cell membranes so that pigment can pass through them HYPOTHESIS: On higher temperatures proteins in membranes denature so there is no barrier to prevent the passage of large molecules THEORETICAL BACKGROUND: Certain chemicals and treatments

The Effects of temperature on Enzymes

733 words - 3 pages The Effects of Temperature on Enzymes Reaction Rate. INTRODUCTION Enzymes are proteins that speed up many chemical reactions in cells. Enzymes affect the rate of almost all chemical reactions which take place in organisms. Like all other proteins, enzyme function may be affected by the change in any aspect of its structure. The effect of a change in enzyme structure is called denaturation; this means that the enzyme becomes inactive

The Effect of Using Social Networking Sites on Acedemic Performance of Secondary and Tertiary Level

1952 words - 8 pages THE EFFECT OF USING SOCIAL NETWORKING SITES ON ACEDEMIC PERFORMANCE OF SECONDARY AND TERTIARY LEVEL ____________________ A Thesis Proposal Presented to the Faculty of the College of Information and Communications Technology Bulacan State University Malolos City ____________________ In Partial Fulfillment of the Requirements for the Degree Bachelor of Science in Information Technology


933 words - 4 pages solar still. As the water depth increase there is no significant change in the convective and radiative heat transfer coefficient. Evaporative heat transfer coefficient depends on the depth of water due to the increase in water temperature as the depth decreases. Finned Velmurugan et al [5] investigated the effect of introducing fins inside the solar still. The introduction of fins in the solar still increases the free surface area of the

Side Effect of Using Smart Phones

660 words - 3 pages will go back to their own room and continue playing with their smartphones again , the mother is left cleaning and washing dishes alone in the kitchen . Therefore , they will never speak to each other . So , smartphone will weaken the relationship of the family members. Another side effect of using smartphone is it causes people become more materialistic especially teenagers today . Teenagers like to compare property to their friends such as

The Cause and Effect of Water Pollution in Lake Huron

1156 words - 5 pages them. Next, entrepreneurs have to consider funding which is among the most difficult challenges at start-up. Small businesses in particular have the option of using personal resources stemming from family, friends, and credit cards. These options can be highly risky seeing that with family and friends, it can be extremely difficult to maintain a professional relationship. With credit cards, business owners can gain access to fast and flexible

Effect of Culture on Communication

3260 words - 14 pages alive and very common in South Africa (Ramsingh & Dobie 2009)). This communication misunderstanding can be explained using the long-term orientation dimension of culture. People who originate from mainland China have a very high long-term orientation score, linked to their historic Confucian Dynamism (see blue LTO column in Graph- 4 below). Core Asian values stemming from the Chinese philosopher Confucius, due to their focus on long term

Related Essays

Effects Of Different Ph Level On Amylase In Starch

2961 words - 12 pages . Literature cited Butterworth, P. J., Frederick W. J., and Peter E. R. (2011). “Human α-amylase and Starch Digestion: An Interesting Marriage” Starch. 63(7), 395-405.  Goto, S., Nishioka, T., Kaneshisa, M. (1998). “LIGAND: chemical database for enzyme reactions.” Bioinformatics. 14 (7): 591-599. Guyot, J.P., Calderon, M., and Morlon-Guyot, M. (2000). “Effect of pH control on lactic acid fermentation of starch by Lactobacillus

Effect Of Enzymes On Starch Essay

2133 words - 9 pages (mm) 15 38.8 40 50 Standard Deviation 0 ± 3.03 ± 1.87 ± 4.12 Qualitative data Amylase is milky white in color Petri Dishes contains condensed vapor on the lid Lots more condensed vapor after taking out of incubator Majority of the area is dark purple in color after pouring iodine on the agar Sample Calculations Average diameter The aim of this lab is to compare the results of different concentrations of amylase to starch. In

The Efeects Of Salivary Amylase On Starch Hydrolysis Under Different Ph Levels

746 words - 3 pages into the tube.The enzyme collected in this experiment is salivary amylase from the mouth. Which was obtained by having one individual in a group chew on a small piece of Parafilm wax paper. The first 2-3mL of saliva were discarded since they contain more water than the actaul enzyme. The next 5 mL of saliva were placed into a beaker and then diluted with with 15 mL of distilled water.The serial dilution preparation was made using three 125mL flasks

Effect Of Temperature On Raction Rate

684 words - 3 pages flask. 6-     Put the flask in a water bath at a controlled temperature of 30ºC. 7-     Quickly add the second solution and start timing 8-     Stop timing when you can no longer see the mark (X) on the tile. 9-     Repeat steps 1 to 4 but using a different temperature each time. 10-     The final time use the same