This website uses cookies to ensure you have the best experience. Learn more

Detection Of  Staphylococcus Aureus In Chicken Macaroni Salad

1674 words - 7 pages

Detection of  Staphylococcus aureus in Chicken Macaroni Salad

Cardinez, M.C.C.a, Delos Reyes, A.J.J.a, Jacosalem, G.M.a, Quiatchon, P.A.N.a, Tumambing, V.K.Ma

a Department of Food Science and Nutrition, College of Home Economics, University of the Philippines, Diliman, Quezon City 1101, Philippines

1. INTRODUCTION
The macaroni salad is a pasta salad served cold usually made from elbow macaroni and mayonnaise as its dressing. Like potato salad and coleslaw, macaroni salad is commonly presented as a side dish of barbecued chicken and meat meals. However, in the Philippines, the macaroni salad is served as a dessert because of its sweeter taste compared to other macaroni salads ...view middle of the document...

Staphylococcus, which is a Gram-positive, catalase positive bacteria have been well known to cause several infections to human. Some of these include pimples, boils, pneumonia, food-borne gastroenteritis, wound infections and nosocomial or hospital-associated infections (J.M.Jay, 2005, Tortora, et.al. 2007). Some more extraintestinal infections of Staphylococcus to human are burn infections, impetigo, pseudomembraneous colitis, scalded skin syndrome, bacteraemia, meningitis and toxic shock syndrome (Varnam, 1996).
The antimicrobial properties of penicillin against Staphylococcus had been a success until the 1950's when hospitals were bothered by the emergence of penicillin- resistant strains of Staphylococcus and lead to use of methicillin. In 1980, methicillin- resistant S. aureus emerged and required the use of yet another antimicrobial known as vancomycin and in 2002, a case of vancomycin- resistant S. aureus was recorded (Tortora, et.al. 2007).
Ranking 1 as the microorganism which caused most number of nosocomial infections was coagulase-negative I which caused 25 % of sepsis reported, 89% of this is resistant to antibiotics. It caused 16% of pneumonia cases, where 60% are antibiotics- resistant (Tortora, 2007).
This study was conducted to determine the presence of Staphylococcus aureus in ready-to-eat foods like the home-made macaroni salad and to discern the factors that led to the contamination of this food with this microorganism.

2. MATERIALS AND METHODS
2.1    Acquisition of food sample
        The food sample used was chicken macaroni salad which was prepared at home. During the preparation of the food sample, the ingredients had a lot of contact with bare hands. The stripping of the chicken meat, slicing of cheese and ham and washing of the macaroni were all done with bare hands.  After preparation of the macaroni salad, it was stored in the refrigerator at -5°C for 5 days. The sample was placed in a plastic bag and brought to the laboratory for analysis.

2.2    Determination of Staphylococcus aureus
        Approximately 25 g of chicken macaroni salad was randomly obtained as food sample using a triple beam balance (Ohaus, 700/800 Series, USA). The food sample was then homognized with 225 mL of sterile Peptone Water (PW) (HiMedia, HiMedia Laboratories, India) using a sterile blender (Moullinex Type Q48, Mexico) for 60 sec at medium speed to make the 10-1 dilution. Ten-fold serial dilutions upto 10-3 were prepared by aseptically obtaining 1 mL from the homogenate and mixing with 9 mL of PW.
        The dilutions were then inoculated by spread plating 0.4 mL of the each dilution onto the pre-solidified Baird-Parker Agar with Egg Yolk Tellurite using a sterile glass hockey stick. The Baird-Parker agar with egg yolk Tellurite was prepared by mixing 8.84 mL egg yolk Tellurite solution into 150 mL Baird-Parker agar(HiMedia, HiMedia Laboratories, India). Inoculation was done in duplicates. A control plate with Baird-Parker...

Other Papers Like Detection of  Staphylococcus Aureus in Chicken Macaroni Salad

Renal Diet Chart Essay

5055 words - 21 pages . Below is a list of items to assist you in choosing sensibly from any salad bar. 11 Fast Food Facts continued... Include these items in your salad bar choices: Alfalfa Sprouts Beets Cauliflower Celery sticks Chinese Noodles Cole Slaw Cucumbers Eggs, chopped Gelatin salads Green beans Green peas Green peppers Italian, low calorie dressing Lettuce, escarole, endive Macaroni salad Mushrooms Oil and vinegar dressing Okra Onions Parmesan cheese

Food Safety Knowledge And Practices Essay

8468 words - 34 pages Knowledge Posttest (85.8% correct) asked participants which food--a slice of toast, macaroni salad, or gumbo--was the least likely to cause illness from microorganism growth. This question may have been misleading or perhaps this topic was not adequately emphasized in the curriculum. 40 Food Safety Practices Survey Results from the Food Safety Practices Survey administered immediately after the food safety curricular instruction

Apertizer

1427 words - 6 pages pickles and mint leaves in vinegar dressing. Topped with shredded chicken breast, chopped peanuts and dried onions. 10. Spicy Lemon Beef Salad Spicy Lemon Beef Salad (Bò Tái Chanh)$10.95 Basil, mint, lettuce, cucumber, Tomato and sliced onions. Topped with thinly sliced medium rare beef dipped in Lemon juice. SOUPS 11. Asparagus Crab Soup (Soup Măng Tây Cua)$6.95 Asparagus in chicken and pork broth with crab meat. 12. Oxtail Soup (Soup Đuôi

Instrumental Learning Paper

982 words - 4 pages The learning situation that I will discuss is my evolution of making macaroni and cheese. Growing up the youngest of four girls I never had the chance to really cook for my family because it was always done for me. As a child I always wanted to participate in holiday dinners and I finally got the chance to when I was thirteen. My job was to make the macaroni and cheese for Thanksgiving. The goal of making macaroni and cheese is to make it tasty

Patient Falls

720 words - 3 pages Nursing a patient with MRSA Introduction. I have been asked to write a reflection about nursing a patient with MRSA in a hospital. In my writing I am going to explain what is meant my term MRSA and how it is originated,spread,treated and controled. MRSA stands for Methicillin Resistant Staphylococcus Aureus. This term used to describe about the bacteria which are resistant to number of antibiotics including Methicillin. Staphylococcus

Salad Lovers

547 words - 3 pages SALAD LOVERS When I was a child a salad was a very important part of our meal. My father was always the one in charge of preparing the salads. No matter what day it was, whether it was Christmas, birthdays, or just a simple meal, he managed to make the most outstanding salad. He was so creative and put so much love in making them. Is it any wonder I have celestial standards when it comes to the perfect salad? In my early years, as I was

Micro Quiz 4

1520 words - 7 pages superabsorbent tampons left in too long, allowed for the multiplication of strains of Staphylococcus aureus producing ecotoxin C (c) Infection of the prostate in men. commonly caused by Escherichia coil (d) An immune complex disease of the glomeruli of the kidney tipped of by nephrogenic strains off Streptococcus pyogenes (e) An inflammation of the kidney usually caused by the backup of urine with consequent ascent of Escherichia coli (f) A

Food Borne Pathogens

1370 words - 6 pages , fever, headache, chills, and prostration. The possible contaminant is meat, poultry, egg or milk products. Cooking thoroughly; avoiding cross-contamination; and using sanitary practices are the best way to prevent contamination.   STAPHYLOCOCCUS The cause of illness is toxin produced by certain strains of Staphylococcus aureus, it has a incubation period of one to six hours. Severe vomiting, diarrhea, abdominal cramping are the symptoms

Wound Healing Activity of Tawatawa

3577 words - 15 pages antibacterial potential of decoction preparations as commonly used in medication. Hence, the goal of this study is to assess the safety of tawa-tawa (Euphorbia hirta L.), decoction as alternative folkloric medicine by evaluating its antibacterial activity against several gram-positive (Staphylococcus aureus; Bacillus subtilis) and negative (Escherichia coli; Pseudomonas aeruginosa) bacterial strains in vitro, that are commonly associated with humans

Pasta E Fagioli

307 words - 2 pages | 1 teaspoon dried rosemary, or 1 tablespoon chopped fresh rosemary | 6 cups drained and rinsed canned kidney beans (three 19-ounce cans) | 2 3/4 cups canned low-sodium chicken broth or homemade stock | 1 bay leaf | 1 teaspoon salt | 1/2 cup tubetti or other small macaroni | 1/2 teaspoon fresh-ground black pepper | | | | | | DIRECTIONS In a large pot, heat 1 tablespoon of the oil over moderate heat. Add the

Journel Article Review

837 words - 4 pages biomaterial inside the body strongly increases the chances at developing a biomaterial-associated infection caused by staphylococci—specifically Staphylococcus epidermidis and Staphylococcus aureus. The hygiene measures, or lack thereof, are what cause the infections to take place. The patient’s skin and surgical equipment carry bacteria, and if these bacteria adhere to the device being implanted, a biofilm (is any group of microorganisms in

Related Essays

Mrsa Community Essay

935 words - 4 pages Clinical significance MRSA is a strain of Staph Aureus which does not respond to treatment from antibiotic drugs such as cefoxitin and methicillin (1), (2). This is generally associated as an organism which colonises patients in a hospital setting. It can cause infections from soft tissue infections to sepsis, pneumonia, tissue damage and necrosis. Community acquired MRSA is not just a hospital acquired strain that has escaped into the

Laboratory Diagnosis Of Respiratory Infections Essay

1568 words - 7 pages antigen has a sensitivity of 80% at our institution and very good specificity but numbers or low so data should be considered carefully; test can remain positive for months nosocomial infections are rare at our institution; only know of 1 documented nosocomial case since I have been here d. Staphylococcus aureus- important CF pathogen, CAP secondary to influenza, nosocomial infection; CA-MRSA found in 3% of CF patients in a 2 year

Abc Project Essay

1077 words - 5 pages contribution margin menu engineering (CM ME) analysis by using the data displayed in Table 2. ABC Stage Two 5. Trace overhead costs from the activity cost pools to the “Garden Burger,” “ Chicken Caesar Wrap,” and “ Cobb Salad” items, and construct “bills of activities” for these items (resource consumptions for the items are displayed in Tables 3, 4 & 5). 6. Calculate the ABC costs for all 3 items and conduct an ABC menu engineering

Mrsa Infection Essay

1163 words - 5 pages Staphylococcus aureus bacteria, typically live in nasal passages and on the skin of healthy individuals, and enter the body through a cut, catheter or breathing tube during a stay in a hospital. Potentially life-threatening MRSA infections most often occur within health care settings (CDC, 2011). Septic shock (result of a severe infection) can occur as a result of the body’s inflammatory response to toxins released by the MRSA infection. The inflammatory