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Chromotography Essay

3895 words - 16 pages

Chromatography
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Pictured is a sophisticated gas chromatography system. This instrument records concentrations of acrylonitrile in the air at various points throughout the chemical laboratory.
Chromatography (from Greek χρῶμα chroma "color" and γράφειν graphein "to write") is the collective term for a set of laboratory techniques for the separation of mixtures. It involves passing a mixture dissolved in a "mobile phase" through a stationary phase, which separates the analyte to be measured from other molecules in the mixture based on differential partitioning between the mobile and stationary phases. Subtle differences in ...view middle of the document...

3 Simulated moving-bed chromatography * 8.4 Pyrolysis gas chromatography * 8.5 Fast protein liquid chromatography * 8.6 Countercurrent chromatography * 8.7 Chiral chromatography * 9 See also * 10 References * 11 External links |
[edit] History
Main article: History of chromatography
The history of chromatography begins during the mid-19th century. Chromatography, literally "color writing", was used—and named— in the first decade of the 20th century, primarily for the separation of plant pigments such as chlorophyll. New types of chromatography developed during the 1930s and 1940s made the technique useful for many types of separation process.
Some related techniques were developed during the 19th century (and even before), but the first true chromatography is usually attributed to Russian botanist Mikhail Semyonovich Tsvet, who used columns of calcium carbonate for separating plant pigments during the first decade of the 20th century during his research of chlorophyll.
Chromatography became developed substantially as a result of the work of Archer John Porter Martin and Richard Laurence Millington Synge during the 1940s and 1950s. They established the principles and basic techniques of partition chromatography, and their work encouraged the rapid development of several types of chromatography method: paper chromatography, gas chromatography, and what would become known as high performance liquid chromatography. Since then, the technology has advanced rapidly. Researchers found that the main principles of Tsvet's chromatography could be applied in many different ways, resulting in the different varieties of chromatography described below. Simultaneously, advances continually improved the technical performance of chromatography, allowing the separation of increasingly similar molecules.
[edit] Chromatography terms
* The analyte is the substance to be separated during chromatography.
* Analytical chromatography is used to determine the existence and possibly also the concentration of analyte(s) in a sample.
* A bonded phase is a stationary phase that is covalently bonded to the support particles or to the inside wall of the column tubing.
* A chromatogram is the visual output of the chromatograph. In the case of an optimal separation, different peaks or patterns on the chromatogram correspond to different components of the separated mixture.

Plotted on the x-axis is the retention time and plotted on the y-axis a signal (for example obtained by a spectrophotometer, mass spectrometer or a variety of other detectors) corresponding to the response created by the analytes exiting the system. In the case of an optimal system the signal is proportional to the concentration of the specific analyte separated.
* A chromatograph is equipment that enables a sophisticated separation e.g. gas chromatographic or liquid chromatographic separation.
* Chromatography is a physical method of separation in which...

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