* To investigate the structure of a cell membrane
The cell membrane is made up of the phospholipid bilayer which is infused with lipids and proteins which keep the membranes structure intact. Acids have properties which allow them to react with the lipids/ proteins- specifically the polypeptide chain; denaturing them which causes the membrane to break. This cause the organelles (such as pigment) in the cells in the membrane are released.
* How does the concentration of HCl affect the structure of the cell membrane of beetroot, measured by light transmittance through the HCl/beetroot solution?
* As the ...view middle of the document...
Access pigment on Beetroot | Wash beetroot after cutting into relative size in water |
Surface area of the cut beetroot samples | Cut into relative sizes and weigh on electric scale so they have the same mass |
List all of the equipment you are going to use in the investigation.
* 2 Knives
* Cutting Board
* 26 sample bottles with lids
* 26 curvets and lids
* Logger pro programmed computer
* 3 Beetroot
* 500mL of distilled water
* 500mL of HCl
* 200mL Beaker x3
* 20mL measuring cylinder
* 10mL measuring cylinder
* Ice cream container
* Electronic balance
* Paper towels
1. Peel and cut up 3 beetroot to 26 pieces of relative sizes of 1cm x1cm x2cm that fit into the curvet
2. Put paper towel on scale and calibrate
3. Insure all 26 pieces are relatively the same mass by measuring on an electronic balance and cutting so all mass are 2g~4g.
4. Clean Beetroots dab them dry with paper towel and put each in a curvet
5. Label curvet with 0.0%, 12.5%, 25.0%, 50.0%, 100.0%
6. Pour 1 mole-1 HCl in 100% curvet up to the line.
7. Pour distilled water in 0.0% curvet up to the line.
8. Mix 5mL of HCl with 5mL of distilled water to make 50%
9. Pour mixture up to the line, into 50% labelled curvet
10. Mix 5mL of HCl with 15mL of distilled water to make 25%
11. Pour mixture up to the line, into 25% labelled curvet
12. Mix 5mL of HCl with 20mL of distilled water to make 12.5%
13. Pour mixture up to the line, into 12.5% labelled curvet
14. Repeat whole process from 1 to 12, five times so that there are 25 samples in total
15. Close all curvets and leave for 24 hours in an ice cream container in a cupboard
16. Connect colorimeter to laptop with logger pro
17. Clean curvets with paper towels and dry
18. Pipette clean
19. Wait 5 minutes for the colorimeter to warm up
20. Pour distilled water into curvet with pipette and put on lid.
21. Insert into colorimeter and press calibrate
22. Wait 3 seconds and record the calibration
23. Take out existing curvet in colorimeter
24. Pour in 5 samples of 0.0% concentration solution into 5 different curvets.
25. Shake solution inside curvet after putting the lid on and put into colorimeter and press cal
26. Wait 3 seconds and collect data.
27. Repeat no#23 to no#26 for the rest of the ranges 12.5%, 25.0%, 50.0%, 100.0% of concentration for all 25 samples
28. Study the graph made
Raw Data Table
The raw data table showing the density of the transmittance of light in relation to the concentration of HCl in beetroot/HCl solution. |
Concentration of HCl in solution (±0.1%) | Percentage of Light transmittance through solution (%) |
| Trial 1 | Trial 2 | Trial 3 | Trial 4 | Trial 5 |
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